Grade 9 · Genetics and Heredity · Inheritance of Traits

Punnett Squares and Probability

BiologyCore30 min

Predicting inherited traits using probability.

Learning objectives

  • Complete a monohybrid Punnett square.
  • Predict offspring ratios from parent genotypes.
  • Explain why predictions are probabilities, not guarantees.

NGSS alignment

  • HS-LS3-3

    Apply concepts of statistics and probability to explain the variation and distribution of expressed traits in a population.

  • MS-LS3-2

    Develop and use a model to describe why asexual reproduction results in offspring with identical genetic information and sexual reproduction results in offspring with genetic variation.

MYP criteria

  • Criterion AKnowing and Understanding
  • Criterion CProcessing and Evaluating

ENGAGE

Two brown-eyed parents, one blue-eyed child

Two brown-eyed parents can have a blue-eyed child, but two blue-eyed parents almost never have a brown-eyed child.

Think about it

  • What does this tell you about which allele is hidden?
  • How could you show all the possible combinations?

EXPLAIN

Using a Punnett square

A dominant allele, written as a capital letter, shows in the phenotype whenever it is present. A recessive allele shows only when both alleles are recessive.

A Bb by Bb cross

Bb
BBBBb
bBbbb
  • Genotype ratio 1 BB : 2 Bb : 1 bb
  • Phenotype ratio 3 brown : 1 blue
  • Probability of a blue-eyed child is 1 in 4, or 25 percent

Common misconception

A 3:1 ratio does not mean exactly one in four children will be blue-eyed. Each child is an independent 25 percent chance.

Homozygous means both alleles are the same, BB or bb. Heterozygous means they differ, Bb, which makes the individual a carrier of the recessive allele.

INVESTIGATION

Modelling inheritance with coins

research question

How closely does a coin model match the predicted 3:1 phenotype ratio?

hypothesis

The larger the number of trials, the closer the results will get to 3:1.

variables

Independent: number of trials (10, 50, 200). Dependent: ratio of dominant to recessive phenotypes. Controlled: same coins, same rules.

method

Let heads be B and tails be b. Flip two coins to represent one offspring and record the genotype. Repeat for each trial count.

safety

No significant hazards.

analysis

Compare each observed ratio with 3:1 and explain why sample size matters when predicting inheritance.

Watch

Monohybrids and the Punnett Square Guinea Pigs

Amoeba Sisters · 8 min

Works through Punnett squares step by step.

Before you watch: What ratio do you expect from Bb x Bb?

  1. Complete a Bb x bb cross.
  2. Why are the results probabilities?
Open on YouTube

Interactive simulation · PhET

Natural Selection

Add a dominant allele mutation and watch the proportions of each genotype over generations.

While you explore

  1. Which genotypes show the dominant phenotype?
  2. How do allele proportions change over generations?
Open full screen on PhET

Key vocabulary

Heterozygous
Having two different versions of a gene.
Dominant allele
The version of a gene that shows even if only one copy is present.

Practice questions

0/2 correct

Level 1 · Criterion A

In a Bb x Bb cross, what is the probability of a recessive phenotype?

Level 2 · Criterion A

An individual with two identical alleles for a gene is described as:

MYP criterion tasks

Level 3 · Criterion C

Two heterozygous parents have four children, all with brown eyes. Does this disprove the 3:1 prediction? Justify your answer.

Reflect & track

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